Vacuum Test of Cavity with Liquid Nitrogen

Preservation of T2 Bacteriophage with Liquid Nitrogen.

There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially pur...

DNA Extraction of Almond without Phenol and Liquid Nitrogen

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

Cavity field in liquid dielectrics

Out of the liquid-into the vacuum.

Dear Reader, The first Volume of Biointerphases in March 2006 had an editorial by Greg Exarhos, then chair of the Publication committee of the American Vacuum Society (AVS), and me, entitled: A surface scientist’s perspective on Biointerphases, or “Out of the Vacuum, into the Liquid” (the latter title I used in many talks and presentations). There we summarized the reason why we were starting a...

Hazards of Liquid Nitrogen Cryovials

There have been several recent incidents involving the exploding of cryopreservation vials in Trust and Medical School laboratories. This occurs when liquid nitrogen (LN ) leaks into the vials and rapidly expands when removed from the tank. In one incident in our Medical School a research worker’s hand was injured. At Edinburgh University the lid of a cryovial was projected into a student’s eye...